Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Presence of Borrelia burgdorferi sensu lato antibodies in the serum of patients with abdominal aortic aneurysms

doi: 10.1007/s10096-011-1375-y

Figure Lengend Snippet: Seroprevalences in different risk groups in Germany [ – ]. Only studies with both enzyme-linked immunosorbent assay (ELISA) and immunoblotting data were included. The confidence intervals for binomial probabilities were calculated in R

Article Snippet: Borrelia afzelii + VlsE IgG ELISA (Pko strain) and Borrelia afzelii IgM ELISA test kits (Genzyme Virotec GmbH, Rüsselsheim, Germany) were used as screening tests in the serum of 96 AAA patients and 108 PAD patients to quantify specific IgG and IgM classes.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

Journal: European Journal of Clinical Microbiology & Infectious Diseases

Article Title: Presence of Borrelia burgdorferi sensu lato antibodies in the serum of patients with abdominal aortic aneurysms

doi: 10.1007/s10096-011-1375-y

Figure Lengend Snippet: Summary of immunoblotting results for abdominal aortic aneurysm (AAA) and peripheral artery disease (PAD) patients

Article Snippet: Borrelia afzelii + VlsE IgG ELISA (Pko strain) and Borrelia afzelii IgM ELISA test kits (Genzyme Virotec GmbH, Rüsselsheim, Germany) were used as screening tests in the serum of 96 AAA patients and 108 PAD patients to quantify specific IgG and IgM classes.

Techniques: Western Blot

Journal: Frontiers in Immunology

Article Title: Chemokines and chemokine receptors in Behçet’s disease

doi: 10.3389/fimmu.2023.1109147

Figure Lengend Snippet: Chemokines and chemokine receptors with potential for BD laboratory biomarkers.

Article Snippet: In addition, many strategies that target the chemokine–chemokine receptor system, including some small molecule blockers and antibodies [the CXCL10 antibody (MDX-1100), CCL2 antibody (Genzyme Techne) and CXCR4 inhibitor (AMD3100)] for common autoimmune diseases (such as rheumatoid arthritis, systemic sclerosis and systemic lupus erythematosus), have been developed and studied in preclinical and clinical trials with effective outcomes ( ).

Techniques: Biomarker Discovery, Activity Assay, Clinical Proteomics

Journal:

Article Title: IL-18 contributes to the spontaneous development of atopic dermatitis-like inflammatory skin lesion independently of IgE/stat6 under specific pathogen-free conditions

doi: 10.1073/pnas.152337799

Figure Lengend Snippet: Spontaneous deviation of splenic CD4+ T cells into Th2 cells. (a) High serum levels of IgE and IgG1 in both types of transgenic mice. Sera were sampled from the various types of mice (36 weeks old) and their serum levels of various types of Ig were measured by ELISA. Data are represented as mean ± SD of triplicate cultures. Similar results were obtained in three independent experiments. (b and c) Spontaneous development of Th2 cells in both types of transgenic mice. Splenic CD4+ T cells from KCASP1Tg or WT littermates at 12 weeks of age (b) or from KIL-18Tg or WT littermates at 36 weeks of age (c) were isolated by membrane attack complexes and were incubated with immobilized anti-CD3 for 48 h. Concentration of IL-4 and IFN-γ in each supernatant was determined by ELISA. Data are represented as mean ± SD of triplicate cultures. Similar results were obtained in three independent experiments. (d) Increase of CD40L-expressing CD4+ T cells in both types of transgenic mice. Spleen cells were isolated from KCASP1Tg, KIL-18Tg, or WT littermate at 36 weeks of age, and their CD40L expression gated on CD4+ cells were identified by flow cytometry. A representative result is shown. Similar results were obtained in three independent experiments. ND, not detected.

Article Snippet: IL-4 and IFN-γ levels were measured by corresponding ELISA kits (Genzyme TECHNE).

Techniques: Transgenic Assay, Enzyme-linked Immunosorbent Assay, Isolation, Incubation, Concentration Assay, Expressing, Flow Cytometry